Our group is interested on understanding lipid diversity and uncovering new lipid functions in cell biology with a focus on membrane trafficking events. We analyze the lipidome of cells using mass spectrometry techniques to uncover new networks of co-regulated lipids that help us identify new lipid roles in cells (Jiménez-Rojo, Leonetti et al, 2020). We combine this with cell and chemical biology approaches that allow us to engineer the lipid composition of cells and thus to manipulate biophysical properties such us membrane fluidity in vitro and in cellula (Jiménez-Rojo, Feng, Morstein et al, 2022). We also apply quantitative microscopy techniques to study membrane trafficking events such as the RUSH assay or Retention using Selective Hooks (Boncompain et al, 2012). We are also studying the role of lipids and membrane mechanics in secretion phenotypes related to cellular senescence and ageing in order to establish biological membranes and lipids as direct contributors to the ageing phenotype.